Northern B.C. salmon are the focus of a new study that may revolutionize how we count seasonal runs, and expand our understanding of populations in streams too remote to include in todaysa国际传媒檚 surveys.
The study is a collaboration between Simon Fraser University and the Gitanyow Fisheries Authority (the technical arm of the Gitanyow Hereditary Chiefs near Terrace) with the goal of determining if salmonid environmental DNA (eDNA) found in river-water samples can establish the number of spawning fish as accurately as manual counts.
sa国际传媒淚 think wesa国际传媒檒l be successful. Wesa国际传媒檙e looking at five different species and I think wesa国际传媒檝e got a really good chance at this,sa国际传媒� said lead researcher, SFU biology professor Vicki Marlatt.
Environmental DNA refers to traces of DNA animals shed into their surroundings sa国际传媒� a pink salmon, for example, will release billions of cells per day in its feces and dislodged scales. For this study, researchers will collect one-litre samples of river water on a daily basis then count the DNA occurrences of the targeted species. With the help of a statistician, whatsa国际传媒檚 found in that sample can be extrapolated to account for the entire stream.
sa国际传媒淭heresa国际传媒檚 a fair bit of math behind it,sa国际传媒� Marlatt said.
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The challenges are to figure out exactly how many cells each salmon species sheds in one day, to avoid counting an individual fish more than once, and then calibrating the process to account for ever-changing river conditions. But if the math is right, the count will be equal to the conventional manual method, where salmon are tallied one by one as they pass through fences and gates spanning the river.
The benefit of eDNA sampling means a low-cost, non-invasive method of counting that doesnsa国际传媒檛 require constant human presence. It can also be expanded for wider commercial use across fisheries and aquaculture sectors.
sa国际传媒淚f wesa国际传媒檙e successful, this would allow us to collect more data on the number of salmon in hundreds of rivers that are not being counted in Canada,sa国际传媒� Marlatt said. sa国际传媒淎lso, if this proves to be more effective and efficient, in terms of human time and cost, then certainly this is something we could consider replacing some of the fish fences with.sa国际传媒�
But eDNA sampling would augment, not replace, the fence counts on the Kitwanga River.
Gitanyow Fisheries head biologist and senior technical advisor, Mark Cleveland, explained the fence off the mid-point of the Skeena River is the only one among few in the Northwest that counts all five species of salmon. Itsa国际传媒檚 a state-of-the-art device with a high-definition camera to assist with manual counts that also enables staff to isolate about five per cent of the passing fish for important physical inspections for weight, sex and overall health.
sa国际传媒淭his is a unique facility sa国际传媒� but itsa国际传媒檚 really labour intensive and expensive so you cansa国际传媒檛 have them everywhere,sa国际传媒� Cleveland said.
sa国际传媒淲ith this eDNA technology, 10 years down the road we might have it dialed in so we can go take water samples in different watersheds sa国际传媒� if you take it at key times, you could do more surveys in more areas and it would result in better management.sa国际传媒�
Accurate counts are critical for Fisheries and Oceans Canada to determine allowable harvests each year in the commercial, recreational and First Nations fisheries. The department will sometimes rely on fence counts when river conditions disrupt efforts at the Tyee Test Fishery in the lower Skeena.
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sa国际传媒淚tsa国际传媒檚 important to reach that balance where wesa国际传媒檙e escaping enough fish to the various places so we donsa国际传媒檛 over-exploit them to the point they donsa国际传媒檛 come back. These programs give us the spot check we need,sa国际传媒� Cleveland said.
The eDNA study was scheduled for completion this year but high rains forced its postponement to 2021.
Marlatt is confident of the experimentsa国际传媒檚 success next year, citing one of very few related studies recently completed in Alaska.
sa国际传媒淚t really is a lot of work, and it was going so well,sa国际传媒� Marlatt said. sa国际传媒淲e managed to get this up and running despite a pandemic and despite getting all the funds laid down and the team together sa国际传媒� then the heavy rains came and the fence had to be opened otherwise the [river] would have taken it down. But wesa国际传媒檙e ready to go next year and Isa国际传媒檓 pretty optimistic, based on the study in Alaska. With some tight manual counts and tight water-flow data, I think wesa国际传媒檒l make some big strides.sa国际传媒�
The study is funded through Genome BCsa国际传媒檚 Sector Innovation Program.
